Selective Dna Sensing Elements Integrated into Microfluidic Channels
نویسنده
چکیده
This paper describes the development of a unique fluorescence based DNA diagnostic assay that does not require labeling of the target sequence prior to analysis. The assay uses polyacrylamide hydrogel plugs immobilized in microfluidic channels, and is based on the displacement of a short sacrificial fluorescent-tagged indicator oligomer by a longer untagged target sequence as it is electrophoresed through the hydrogel plug sensing platform. The distinct advantages of this assay are the short sensing times as a result of directed electrophoretic transport of target DNA to the sensing element combined with the ability to detect non-labeled target DNA.
منابع مشابه
Selective DNA screening in microfluidic channels by electrophoresis through hydrogel matrices
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